Peroral Immunization of Rats with Escherichia coli Heat-Labile Enterotoxin Delivered by Microspheres
نویسندگان
چکیده
منابع مشابه
Heat - labile Enterotoxin of Escherichia coli
Heat-labile enterotoxin (LT) was obtained in large quantities (several-gram amounts) and great purity from Escherichia coli C600 carrying the LT-coding multicopy plasmid EWD299. By growing this strain on a medium that allows high cell densities in the early stationary phase, we increased the net LT production per milliliter by a factor of 200, compared to natural porcine enterotoxigenic E. coli...
متن کاملEscherichia coli Heat - labile Enterotoxin NUCLEOTIDE SEQUENCE
We report the complete DNA sequence of the Escherichia coli elt A gene, which codes for the A subunit of the heat-labile enterotoxin, LT. The amino acid sequence of the LT A subunit has been deduced from the DNA sequence of elt A. The LT A subunit starts with methionine, ends with leucine, and comprises 264 amino acids. The computed molecular weight of LT A is 29,673. The A subunit of cho...
متن کاملRelease of heat-labile enterotoxin subunits by Escherichia coli.
Most of the heat-labile enterotoxin (LT) synthesized by Escherichia coli is cell associated; however, a small portion of LT (approximately 10%) is released by bacterial cells into the culture supernatant. The LT subunit B (LT-B) produced by a cloned LT-B gene (tox B) was released in amounts equal to the parent LT release. In contrast, no release of LT subunit A (LT-A) or its smaller derivatives...
متن کاملImmunization of rats with heat-labile enterotoxin provides uniform protection against heterologous serotypes of enterotoxigenic Escherichia coli.
Rats immunized with Escherichia coli heat-labile (LT) enterotoxin, either in the form of the holotoxin derived from a transformed K-12 strain or the polymyxin-release form obtained from human strains which produce LT toxin alone (LT+/ST- [ST is heat-stable toxin)] or together with ST toxin (LT+/ST+), were challenged with viable organisms of 10 different serotypes, 5 LT+/ST- and 5 LT+/ST+. The s...
متن کاملCellular location of heat-labile enterotoxin in Escherichia coli.
We demonstrated that both the A and B subunits of heat-labile enterotoxin from Escherichia coli are located in the periplasm. The toxin was shown to form aggregates in Tris-EDTA buffers which are routinely used for isolating membranes. The aggregates pellet upon centrifugation, and this may explain why several previous investigators have concluded that enterotoxin is associated with membranes.
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1983
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.39.2.1000-1003.1983